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A formulation of Ocimum sanctum, Phyllanthus emblica, and Glycyrrhiza glabra in a defined ratio termed CIM-Candy was prepared based on Ayurvedic methodology. To study the humoral immune response, rabbit red blood corpuscles (rRBCs) were used as an antigen. Mice were immunized on day 7 and 14 days the start of the experiment using an intraperitoneal injection of 200 μl of rRBCs (2×108 cells/ml). Humoral immune response, body weight variation, and hematology parameters were studied. CIM-Candy at doses of 10 and 100 mg/kg body weight enhanced the immune function by significantly increasing (p<0.05) the hemagglutination antibody titer response against the rRBCs. Significant changes were not observed in body weight and hematology parameters when comparing the normal vehicle control and treatment groups. As the biological activity of the plant-derived formulation was not assigned to a single chemical, high performance thin layer chromatography (HPTLC) fingerprinting was also performed to confirm the chemical characteristic of the formulation. The results of this study suggest that the standardized formulation CIM-Candy exhibited significant humoral immune response in Swiss albino mice.

Immunopotentiating Effect of an Ayurvedic Preparation from Medicinal Plants