English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The effect of bee pollen extract on osteoblas- tic MC3T3-E1 cells was investigated. The water- solubilized extracts, which were obtained from the bee pollen of Cistus ladaniferus ,w aspurified using the membrane fractionation method with molecular weight (MW) less than 1000. Osteoblastic cells were cultured for 72hr in a medium containing either ve- hicle or cistus extract of less than MW 1000 (10, 25, or 50µg/ml of medium) in the presence of 10% fetal bovine serum (FBS). The proliferation of osteoblas- tic cells was significantly enhanced in the presence of cistus extract (25 or 50µg/ml). The stimulatory ef- fect of cistus extract on cell proliferation was also ob- served when the cells with subconfluency were cul- tured for 24 or 72hr without FBS. Cells with subcon- fluency were cultured for 24 or 72hr in a medium containing either vehicle o rc istus extract without FBS. DNA content or alkaline phosphatase activity in osteoblastic cells was significantly increased after cul- turewithcistus extract (50µg/ml) for24hr. The effect of 25µg/ml of cistus extract o nt hesecomponents was seen after culture for 72hr. This study demonstrates that the cistus extract fraction of less than MW 1000 has anabolic effects in osteoblastic MC3T3-E1 cells which involve in bone formation.

Anabolic Effects of Bee Pollen Cistus ladaniferus Extract in Osteoblastic MC3T3-E1 Cells In Vitro