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Glutaraldehyde has been in widespread use in hospitals to sterilize instruments which are not suitable for heat sterilization. Routine exposure to glutaraldehyde is, however, known to cause adverse health effects such as eye irritation, sore throats, skin irritations, dermatitis, short-term memory loss and fatigue, especially for workers in endoscopy, dentistry and other medical departments within hospitals. The American Conference of Government Industrial Hygienists (ACGIH) has set ceiling exposure limit (TLV-C) for glutaraldehyde in workplace atmosphere to be 0.05 ppm. In the U.K., Maximum Exposure Limit (MEL) has been set at 0.05 ppm for both long-term (8 hr) and short-term (15 min) exposure as an occupational exposure limit. In February 2005, the Ministry of Health, Labour and Welfare of Japan has given a notice on the glutaraldehyde usage, in which the monitoring of indoor concentrations of glutaraldehyde is encouraged in sterilization workplaces, and the maximum concentration of 0.05 ppm is recommended. A solid adsorbent coated with 2,4-dinitrophenylhydrazine (DNPH) was previously used and evaluated for the determination of glutaraldehyde in air: DNPH-impregnated glass fiber filters, DNPH coated XAD-2 and DNPH coated silica gel. DNPH-glutaraldehyde derivatives formed when the aldehyde is pumped over the collection media is desorbed by adding acetonitrile and subsequently determined by high performance liquid chromatography (HPLC). Such active sampling methods were also employed as a reference method for evaluating sampling performance of passive samplers developed for monitoring personal exposure and indoor air concentrations. Passive samplers, which employ diffusion process based on Fick’s law and hence do not require power supply or other services, are suitable for monitoring personal or indoor exposure concentrations of glutaraldehyde in the occupational environment. Evaluation of Passive Sampler for Measurement of Glutaraldehyde in Occupational Indoor Air