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vironmental samples, and the calculated 3- methylchloranthrene (3-MC) equivalent concentration (MEQ) was introduced as a new quantitative water quality parameter. The chemical MEQ was calculated by multiplying induction equivalency factor (IEF) to GC-MS analysis data. And biological (bio-) MEQ was calculated by comparing the concentration response curve of the sample with those of the 3-MC calibra- tion curve. Therefore, chemical MEQ is an estimation of the toxic effects of polycyclic aromatic hydrocar- bons (PAHs) and bio-MEQ is the total toxic effects of various CYP1A-inducing chemicals in water samples. In this study, bio-MEQ values of water samples were higher than chemical MEQ values and total PAH con- centrations, indicating that there must be other com- pounds in the water sample effective as inducers of EROD and that the biological activities of mixture com- pounds are mainly due to additive effects. There was a good correlation between bio-MEQ and total PAH con- centration. The difference between bio-MEQ and to- tal PAH concentration was high in downstream areas, i.e., polluted sites. Bio-MEQ calculated based on the enzyme-inducing effect of water samples could give in- formation about the biological potency of water samples caused by PAH-like compounds.

journal of health science

Novel Quantitative Assessment for the Toxic Effect of Polycyclic Aromatic Hydrocarbon-Like Compounds in a Water Environment Using the Ethoxyresorufin O-Deethylase Microbioassay.