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A simple and rapid analytical method of oil-soluble coal tar dyes in cosmetics was established using re- versed-phase TLC/scanning densitometry. Eleven kinds of oil-soluble coal tar dyes were able to be sepa- rated completely on reversed-phase TLC plates by the complementary use of 2 solvent systems. The solvent systems were A; n-hexane/2-butanone solution (5 : 1, v/v), solvent system B; acetonitrile/methanol solution (5 : 1, v/v). Then we measured visible absorption spec- tra of spots developed on the reversed-phase TLC plates by scanning densitometer to identify these coal tar dyes. The proposed method was successfully ap- plied to the identification of oil-soluble coal tar dyes in commercial cosmetics. In this study, using a simple and rapid analysis method, we analyzed 11 kinds of oil-soluble coal tar dyes permitted in cosmetics using reversed-phase TLC, then tried to identify these coal tar dyes by directly measuring the visible absorption spectra of spots of coal tar dyes developed on the TLC plate using scanning densitometer. We report our results in this paper.

Identification of Oil-Soluble Coal Tar Dyes in Cosmetics Using Reversed-Phase TLC/Scanning Densitometry