English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The effect of estrogenic compounds (bisphenol A, genistein, and 17β-estradiol) on the ability of macrophages to produce superoxide and nitric oxide in response to stimulants was investigated. The assays were performed using 96-well microplates to measure multiple samples. Superoxide and nitric oxide were measured by chemiluminescence and fluorescence methods, respectively. Thioglycollate-induced mouse peritoneal macrophages incubated for 24 hr with the estrogenic compounds were stimulated with phorbol 12-myristate 13-acetate and lipopolysaccharide (LPS). The ability of the macrophages to produce superoxide was increased when treated with bisphenol A or genistein, but 17β-estradiol had little effect. Conversely, the ability of the macrophages to produce nitric oxide in response to LPS was strongly suppressed by high concentrations of bispenol A and genistein. 17β-estradiol was ineffective. These results suggest that bisphenol A and genistein similarly affect superoxide and nitric oxide production by a mechanism other than that involving estrogenic activity.

Effect of Estrogenic Compounds on Superoxide and Nitric Oxide Production by Activated Macrophages Assessed by Sensitive Micoplate Assays.