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A method was developed for the simultaneous de- termination of 3 sulfonylurea herbicides, azimsulfuron, flazasulfuron and halosulfuron-methyl in agricultural products. The herbicides were extracted with acetone, re-extracted with ethyl acetate, and then transferred to 2% dipotassium hydrogenphosphate solution. The herbicides were extracted again into ethyl acetate and cleaned up using Sep-Pak® Plus Alumina N and Bond Elut® SAX cartridge columns. The 3 sulfonylurea her- bicides were determined by HPLC. The fortified peaks were confirmed by LC/MS with electrospray ioniza- tion (ESI), and the peaks of azimsulfuron, flazasulfuron and halosulfuron-methyl were determined. The recov- eries of the 3 sulfonylurea herbicides from brown rice, corn, cotton seed, ginkgonut, chestnut, almond, wal- nut, cucumber, pumpkin, orange, grapefruit, manda- rin, lemon and grape ranged from 77.0 to 112.3% fol- lowing fortification at 0.05-0.5 µg/g. The detection lim- its were 0.01 µg/g for azimsulfuron and halosulfuron- methyl, and 0.02 µg/ml for flazasulfuron(S/N > 3).

Simultaneous Determination of Azimsulfuron, Flazasulfuron and Halosulfuron-methyl in Grains, Seeds, Vegetables and Fruits by HPLC.