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Chinese hamster ovary (CHO) K1 cells were treated with 2.5 μM Mitomycin C (MMC) for one hour and incubated in Ham’s F12 medium containing 10% fetal bovine serum or in the medium supplemented with the extract from Cassia nomame pods, leaves and stems. The frequency of cells with chromosome aberrations was significantly lowered by the extract. Proliferation of CHO cells was suppressed by a 1-hr MMC treatment, but the suppression was nullified by the presence of the extract. These findings suggest that the extract can prevent the physiological damage caused by MMC.

The Suppressing Effect of the Extract from Cassia nomame on Clastogenicity and Cytotoxicity of Mitomycin C in CHO Cells.