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A turn-on fluorescent traceable linker based on N-sulfanylethylcoumarinyl amide (SECmide) has been developed as an advanced cleavable linker. It was successfully employed for the enrichment and selective visualization of a target protein in cell lysate. The results demonstrated that the SECmide-based traceable linker is potentially applicable to the identification of low molecular weight target proteins, a factor which has been problematic for a previously developed N-sulfanylethylanilide-based traceable linker.

chemical and pharmaceutical bulletin/chemical and pharmaceutical bulletin

Development of a Turn-On Fluorescent Traceable Linker Employing &lt;i&gt;N&lt;/i&gt;-Sulfanylethylcoumarinyl Amide for the Enrichment and Visualization of Target Proteins