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We describe a new method for detecting hydroxyl radical scavenging capacity based on tris(2,2'-bipyridine)ruthenium(III) [Ru(bpy)(3)(3+)] chemiluminescence and flow injection analysis. Hydroxyl radicals were generated by the Fenton reaction. The scavenging capacity of the six antioxidants tested decreased in the following order: edaravone&gt;L-tryptophan&gt;gallic acid&gt;Trolox&gt;N-acetyl-L-cysteine&gt;ascorbic acid. The proposed method allowed a sample throughput of about 80 samples/h. The six antioxidants were found to inhibit chemiluminescence intensity of Ru(bpy)(3)(2+). The proposed method is a rapid, selective, and accurate procedure for the study of hydroxyl radical scavenging capacity by Ru(bpy)(3)(3+) chemiluminescence.

chemical and pharmaceutical bulletin/chemical and pharmaceutical bulletin

Selective Detection of Hydroxyl Radical Scavenging Capacity Based on Electrogenerated Chemiluminescence Detection Using Tris(2,2'-bipyridine)ruthenium(III) by Flow Injection Analysis