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Simultaneous use of N-hydroxysuccinimide (HOSu) and CuCl2 with a HOSu-based uronium coupling reagent, O-(N-succinimidyl)-1,1,3,3-tetramethyluronium tetrafluoroborate, has been found to eliminate the racemization of the carboxy-terminal N-methylamino acid residue during the segment condensation.

chemical and pharmaceutical bulletin/chemical and pharmaceutical bulletin

O-(N-Succinimidyl)-1,1,3,3-tetramethyluronium Tetrafluoroborate-N-Hydroxysuccinimide-CuCl2: A Facile and Reliable System for Racemization-Free Coupling of Peptides Having a Carboxy-terminal N-Methylamino Acid.