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Silica gel and glass beads were modified using the acid chloride of metal-tetrakis(4-carboxyphenyl)porphine (M-TCPP) through a peptide bond, and an anion-exchange resin was modified with M-TCPP by electrostatic interaction and physical adsorption. The carriers modified with Co -TCPP proved to accelerate the following redox reaction : NADH+O2→^^^(carriers modified with Co-TCPP)NAD++H2O2.The formation of hydrogen peroxide was confirmed by means of a colored reaction. The glass beads medified with Co3+-TCPP exhibited the strongest activity among the tested carriers, and were expected to be useful practically as a solid catalyst for the formation of hydrogen peroxide from NADH.

The Catalytic Activity of Glass Beads, Silica Gels and Anion-Exchange Resins Modified with Metal-Porphines in the Oxidative Reaction of NADH.