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Three new triterpenoidal saponins were isolated from the branches of Calliandra anomala (KUNTH) MACBR. On elucidation of their structures, we noticed correlations between a series of structures and their HPLC retention time. By this behavior and the FAB-MS spectrum, the structures of three saponins (calliandra saponin M, N and O) were established.

Studies on the Constituents of Calliandra anomala (KUNTH) MACBR. IV. Structure Analysis by HPLC Retention Time and FAB-MS Spectrum.