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The synthesis of 19-hydroxylated bile acids (3 alpha,19-dihydroxy-, 3 alpha,7 alpha,19-trihydroxy-, 3 alpha,12 alpha,19-trihydroxy- and 3 alpha,7 alpha,12 alpha,19-tetrahydroxy-5 beta-cholan-24-oic acids) was described. These synthesized 19-hydroxylated bile acids were used as standard samples for the analysis of bile acids in human urine by gas chromatography-mass spectrometry. 3 alpha,7 alpha,12 alpha,19-Tetrahydroxy-5 beta-cholan-24-oic acid was identified in neonatal urines (0.1-1.5 micrograms/ml and 1.5-7% of total bile acids).

chemical and pharmaceutical bulletin/chemical and pharmaceutical bulletin

Synthesis of 19-Hydroxylated Bile Acids and Identification of 3.ALPHA.,7.ALPHA.,12.ALPHA.,19-Tetrahydroxy-5.BETA.-cholan-24-oic Acid in Human Neonatal Urine.