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In order to investigate the chemical characteristics of O6-methylguanines, the substrate of O6-methylguanine-DNA methyltransferase, cis[PtCl(NH3)2(O6,9-dimethylguanine-7)]NO3, a 1:1 complex of cis-platin with O6,9-dimethylguanine, was synthesized and its demethylation rate, pKa, X-ray structure and cytotoxicity were determined.

chemical and pharmaceutical bulletin/chemical and pharmaceutical bulletin

Demethylation of O6-Methylguanine in DNA by O6-Methylguanine-DNA Methyltransferase: Synthesis and Characterization of O6,9-Dimethylguanine Coordinated with cis-Platin at the N7-Position.