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Chemically synthesized (26)- and (27)-methyl-13C-labeled desmosterol (3, 5) were separately incubated with rat liver homogenate. 13C-NMR analysis of the incubation products indicated that the C-25 hydrogen of cholesterol was introduced from the si-face of 3 and the re-face of 5.

Stereochemistry of Hydrogen Addition to C-25 of Desmosterol by Sterol-.DELTA.24-Reductase of Rat Liver Homogenate.