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Both S-(-)- and R-(+)-enantiomers of 5-(p-hydroxyphenyl)-5-phenylhydantoin (p-HPPH), a main oxidative metabolite of the achiral antiepileptic drug phenytoin, could be determined simply, sensitively and accurately using reversed phase high-performance liquid chromatography by using a methanol-monopotassium phosphate eluent containing beta-cyclodextrin. Using this assay procedure, it was determined that an S-(-)-enantiomer was formed predominantly by the oxidation of phenytoin in isolated rat hepatocytes.

chemical and pharmaceutical bulletin/chemical and pharmaceutical bulletin

Optical Separation of Racemic 5-(p-Hydroxyphenyl)-5-phenylhydration by Reversed Phase High-Performance Liquid Chromatography Using Eluents Containing .BETA.-Cyclodextrin.