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During the screening test of the animal crude drug "Chan su" (Chinese name, toad-cake), the venom of Bufo bufo gargorizans CANTOR (Bufonidae), on blood coagulation, platelet aggregation, fibrinolysis system and cytotoxicity, the ethyl acetate extract showed promotive action on platelet aggregation and remarkable cytotoxic activity on HeLa-S3 cells. Nine kinds of bufadienolides were isolated from the ethyl acetate extract by bioactivity-guided fractionation and were identified by chemical and spectral analysis.

chemical and pharmaceutical bulletin/chemical and pharmaceutical bulletin

Hematological Studies on Naturally Occurring Substances. VI. Effects of an Animal Crude Drug "Chan Su"(Bufonis Venenum)on Blood Coagulation, Platelet Aggregation, Fibrinolysis System and Cytotoxicity.