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Glucose oxidase-labeled 11-deoxycortisol was tested for immunoreactivity in an enzyme immunoassay system using a monoclonal antibody. The antigen was labeled by the N-succinimidyl ester method. It was found that the binding affinity of the label to the anti-steroid antibody markedly decreased during storage; the half-life time was ca. 4.5 d.

Unexpected loss of immunoreactivity of a glucose oxidase-labeled antigen in a steroid enzyme immunoassay system.