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A36-residue peptide amide corresponding to the entire amino acid sequence of chicken antral peptide was synthesized by the 9-fluorenylmethyloxycarbonyl (Fmoc)-based solid-phase synthesis, for which a new amide precursor reagent, 3-(α-Fmoc-amino-4-methoxybenzyl)-4-methoxyphenylpropionic acid, was employed in combination with thioanisole-mediated trimethylsilyl bromide deprotection. A homogeneous standard sample prepared by the solution-phase method was used to check the effectiveness of the purification step.

chemical and pharmaceutical bulletin/chemical and pharmaceutical bulletin

Studies on peptides. CLXV. Combination of a new amide-precursor reagent and trimethylsilyl bromide deprotection for the 9-fluorenylmethyloxycarbonyl-based solid-phase synthesis of chicken antral peptide.