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Assignments of labile protons (NH, OH-1, OH-4 and OH-8) and naphthalene carbon atoms in the 1H-and 13C-nuclear magnetic resonance spectra of rifampicin (1) and 3-[(dimethylhydrazono)-methyl] rifamycin SV (2) in CDC13 were made based on long-range selective decoupling experiments. The published assignments of the labile protons and two naphthalene carbon atoms (C-5 and C-9) of 1 should be revised.

1H- and 13C-nuclear magneticresonance spectra of rifampicin and 3-((dimethylhydrazono)methyl)rifamycin SV.thylhydrazono)methyl)rifamycin SV.