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For use in automated synthesis of oligodeoxyribonucleotides, a dimer containing the triesterified internucleotide linkage and the 3 '-phosphoramidite has been prepared. 5 '-O-Dimethoxytritylthymidylyl- (3 '-5') - (o-chlorophenyl) -thymidine was converted to the 3 '- (methyl)N , N-diisopropylphosphoramidite, which has been used as a condensing unit for the synthesis of pentadecathymidylate on controlled pore glass by a deoxyribonucleic acid (DNA) synthesizer.

Blockwise mechanical synthesis of oligonucleotides by the phosphoramidite method.