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Thymosin β4 fragment 16-38, H-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-OH, was synthesized by a solution method, by assembling five peptide fragments followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole in trifluoroacetic acid. The synthetic tricosapeptide was tested for effect on the impaired blastogenic response of phytohemagglutinin-stimulated T-lymphocytes of a uremic patient with pneumonia. The synthetic tricosapeptide was found to have restoring activity, which was approximately equal in potency to that of our synthetic deacetyl-thymosin β4.

chemical and pharmaceutical bulletin/chemical and pharmaceutical bulletin

Synthesis of calf thymosin .BETA.4 fragment 16-38 and its effect on the impaired blastogenic response of T-lymphocytes of a uremic patient with pneumonia.