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Postheparin plasma lipolytic activity consists of hepatic triglyceride lipase (H-TGL) and lipoprotein lipase (LPL) activities. The effect of chlorinated pesticides, pentachlorophenol (PCP) and 1, 1, 1-trichloro-2, 2-bis- (4-chlorophenyl) ethane (DDT), on these lipase activities in rat postheparin plasma was examined. PCP completely inactivated H-TGL but did not change LPL activity. On the other hand, DDT completely inactivated LPL but did not change H-TGL activity. By utilizing these specific inhibitions, a procedure for selective measurement of H-TGL and LPL in postheparin plasma of young and old rats, with and without diabetes, was developed. The values of H-TGL and LPL activity obtained by this method were in good agreement with those obtained by immunochemical methods.

chemical and pharmaceutical bulletin/chemical and pharmaceutical bulletin

Selective measurement of hepatic triglyceride lipase and lipoprotein lipase in rat postheparin plasma with pentachlorophenol and dichlorodiphenyltrichloroethane.