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respiratory chain, depletion and reincorporation experiments were performed. Quinones were removed efficiently by n-pentane-acetone (9 ; 1, v/v) extraction. The depleted membrane showed no reduced nicotinamide adenine dinucleotide (NADH) oxidase activity and yet retained NADH dehydrogenase activity and ubiquinol-1 (Q1H2) oxidase activity. When Q-8 was reincorporated into quinone-depleted membrane vesicles by a freeze-thawsonication method, the NADH oxidase activity was restored completely. However, the other

Indispensability of ubiquinone in the aerobic respiratory chain of Photobacterium phosphoreum.