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N-Salicylidene-4-aminobenzothiazole forms fluorescent complexes with zinc, cadmium and magnesium, which are extractable with isoamylalcohol and the optimum pH for the formation and the extraction of zinc, cadmium and magnesium complexes are 8.9, 10.7, and 8.7, respectively. Especially the fluorescence of the zinc complex is very strong and it is possible to utilize N-salicylidene-4-aminobenzothiazole for the fluorometric determination of zinc in the range of 1.6×10-6M to 0.5×10-8M. Interfering ions except cadmium are suppressed by simple addition of masking agents.

Studies on Benzothiazole Derivatives as Chelating Agents. II. The Fluorometric Determination of Zinc with N-Salicylidene-4-aminobenzothiazole