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In vitro metabolism of 3H-testosterone 17-sulfate(3H-TS)and 14C-testosterone 17-tetrahydropyranyl ether(14C-TP)was studied by incubation with 20000•~g or 105000•~g supernatant and microsomal fractions of male rat liver homogenate under carbon monoxide atmosphere or in air.The results demonstrated that the steroidal ring A of 3H-TS was readily metabolized by microsomal ƒ¢4-5ƒ¿-hydrogenase,while 14C-TP was a good substrate for ƒ¢4-5ƒÀ-hydrogenase present in the 105000•~g supernatant fraction as well as microsomal ƒ¢ 4-5a-hydrogenase.On the other hand,3H-TS and 14C-TP were not good substrates for

<I>In Vitro</I> Metabolism of Testosterone Sulfate and Testosterone Tetrahydropyranyl Ether in Male Rat Liver