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Induction effect of phenobarbital (80 mg/kg, daily for 4 d), 3-methylcholanthrene (40 mg/kg, daily for 4 d), and isosafrole (150 mg/kg, daily for 4 d), on 2- and 4-hydroxylations of estradiol 17-sulfate by rat liver microsomes with an NADPH-generating system was investigated. On catechol formation from estradiol 17-sulfate, only phenobarbital caused some increase of the 2-hydroxylase activity in both sexes. While 4-hydroxylase activity was caused effectively by phenobarbital and isosafrole in both sexes, especially, the induction by the former in male rats was over six-fold increase to the control.

An induction effect of phenobarbital on rat liver microsomal 4-hydroxylation of estradiol 17-sulfate.