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Prostaglandin E1 (PGE1) specifically bound to the membrane prepared from murine renal outer medulla. The extent of binding of [3H]PGE1 to the membrane was increased about 4-fold by guanosine triphosphate (GTP) and its analogs, but the dissociation of bound [3H]PGE1 from the membrane was in turn enhanced by GTP gamma S. Scatchard plot analyses revealed that GTP gamma S increased the binding affinity more than 2-fold without a major change in the number of binding sites. When [3H]PGE1-bound proteins were cross-linked in the membrane by dithiobis (succinimidyl propionate), bound [3H]PGE1 was no longer dissociated by GTP gamma S treatment, suggesting that cross-linking produced a stable complex of PGE receptor with a GTP-binding protein. The cross-linked [3H]PGE1-specifically bound proteins solubilized from the membranes labeled with [3H] PGE1 in the presence or absence of GTP gamma S were eluted as an apparently single radioactive peak at the same position of Mr = 15000 by gel filtration, indicating that the PGE receptor forms a complex with a GTP-binding protein regardless of the treatment with GTP gamma S by which [3H]PGE1 binding is promoted. The ability of GTP gamma S to stimulate [3H]PGE1 binding was eliminated by pretreatment of the membrane with pertussis toxin, but not cholera toxin, indicating that the PGE receptor is coupled to a pertussis toxin-sensitive GTP-binding protein.

Stimulatory effect of guanine nucleotides on prostaglandin E1 binding to murine renal outer medulla.