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A method for the quantitative determination of human serum albumin in plasma and urine by post-column high-performance liquid chromatography with fluorescence detection is presented. Human serum albumin in plasma and urine is separated by gel-permeation column chromatography, which is followed by fluorescence detection. The detection uses the fluorescence enhancement observed when 8-anilino-1-naphthalenesulfonic acid binds to human serum albumin. The method is rapid to perform and is highly sensitive (detection limit is 2.5 ng/injection volume at a signal-to-noise ratio of three.

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High-Performance Liquid Chromatographic Determination of Human Serum Albumin in Plasma and Urine by Post-column Fluorescence Enhancement Detection Using 8-Anilino-1-naphthalenesulfonic Acid.