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Intravenous administration of the carboxamide-methylated light chain (G1L) from human serum IgG induced tumor necrosis factor (TNF), one of the inflammatory cytokines, in the serum of mice. Human serum IgG, however, showed no induction of TNF in the serum. G1L showed a potent anti-inflammatory effect in carrageenin-induced paw edema, and the anti-inflammatory effect of G1L was significantly blocked by the concomitant administration of anti-TNF antiserum, but TNF alone had no anti-inflammatory effect.

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Pharmacological Activity of Chemically Modified Subfragment from Human Serum IgG. XI. Increment of Tumor Necrosis Factor(TNF) Activity in Mouse Sera by Carboxamide-Methylated Light Chain.