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A self-complementary decadeoxyribonucleotide, 5'd(GAAGFGCTTC)3', containing 5-fluorocytosine (F) in substitution for cytosine at the methylation site of DNA-cytosine methylase HhaI (MHhaI) has been synthesized. MHhaI was inhibited by the pre-incubation of the enzyme with d(GAAGFGCTTC).

Nucleosides and Nucleotides. CXIX. Inhibition of DNA-Cytosine Methylase HhaI by a Self-Complementary Oligonucleotide Containing 5-Fluorocytosine.