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Mitochondrial nuclei (mt-nuclei) were isolated from the microplasmodia of Physarum polycephalum in buffer containing polyamines and chelator. The individual mt-nucleus retained the native structure observed in vivo. The DNA of these mt-nuclei was the non-fragmented, native one. These characteristics indicate that the native structure is preserved during isolation. In contast to the preparation isolated with buffer exclusive of polyamines and chelator, the present mt-nuclei were stable during storage and did not aggregate. Thus, they provide useful material for the study of the morphology and biochemistry of the individual mt-nucleus.

Stabilization and Isolation of Mitochondrial Nucleifrom <I>Physarum polycephalum</I>