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A cell line of retinoblastoma cells, TOTL-1, was established which had a very weakly amplified N-myc gene. It grew only in clumps that were hardly ever dispersed and showed retarded proliferation. Its doubling time was 160 h. TOTL-1 cells extended short thick processes in monolayer cultures on poly-D-lysine coated substratum, which were markedly different from those of Y79 cells. Light and electron microscopy observation of immunostaining with anti-pp60src antibody revealed that the staining was accumulated in the polar regions of the cells.

Low-amplified N-myc gene and pp60src in retinoblastoma TOTL-1 cells which undergo neuron-like differentiation.