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The defense toxins, spirostomins A and B, have been isolated as a diastereomeric mixture from the ciliate microorganism Spirostomum teres. The structure of spirostomin was elucidated through a number of NMR experiments which allowed assigning the unprecedented spiro[(2,5-dimethyl- 5,6,7,8-tetrahydronaphthalene-1,4-dione)-8,6′-(pyrane-2′,5′-dione)] skeleton to this natural compound. The total syntheses of the racemic spirostomins confirmed their structure and relative configurations

Spirostomin, Defense Toxin of the Ciliate Spirostomum teres: Isolation, Structure Elucidation, and Synthesis