Open AccessA Proposed Consensus Steroid-Binding Sequence—a Reply
Author(s) -
Stephen E. Fawell,
Jacqueline A. Lees,
Malcolm G. Parker
Publication year - 1989
Publication title -
molecular endocrinology
Language(s) - Uncategorized
Resource type - Journals
eISSN - 1944-9917
pISSN - 0888-8809
DOI - 10.1210/mend-3-6-1002
Subject(s) - biology , consensus sequence , steroid , mutant , receptor , steroid hormone , sequence motif , homology (biology) , sequence (biology) , binding site , estrogen receptor , computational biology , genetics , peptide sequence , microbiology and biotechnology , biochemistry , gene , hormone , cancer , breast cancer
A consensus sequence has been proposed as necessary for steroid binding on the basis of sequence homology in steroidogenic enzymes, steroid binding proteins and steroid receptors. We have mapped the limit of sequences important for steroid binding in the mouse estrogen receptor using Scatchard analysis of deletion mutants. Since a mutant lacking the entire proposed consensus is still able to bind estradiol with essentially wild type affinity, the functional significance of this motif is unclear.
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