Open AccessCharacterization of monoclonal antibodies specific for the human growth hormone 22K and 20K isoforms.
Author(s) -
Mario Mellado,
José Miguel RodríguezFrade,
Leonor Kremer,
Carlos Martínez-Alonso
Publication year - 1996
Publication title -
the journal of clinical endocrinology and metabolism
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.206
H-Index - 353
eISSN - 1945-7197
pISSN - 0021-972X
DOI - 10.1210/jcem.81.4.8636376
Subject(s) - monoclonal antibody , gene isoform , western blot , antibody , microbiology and biotechnology , immunoassay , monoclonal , chemistry , blot , biology , biochemistry , immunology , gene
We have derived and characterized a set of monoclonal antibodies (mAb) specific for the different human GH (hGH) isoforms. The binding characteristics of each antibody to the hGH isoforms (22K and 20K) were analyzed in direct and competitive immunoassays as well as by Western blot. We studied the effects of these mAb on the biological activity of hGH and showed that they specifically block their respective activities. Using these mAb, we developed several immunoassays that have been applied for the quantitation of the different hGH isoforms in body fluids. Therefore, these mAb may help to unravel the biological function of these variants.
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