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Insulin-like growth factor binding proteins (IGFBPs) are produced by many cell types and are present in a wide variety of biological fluids. Detection of IGFBPs in complex fluids is commonly performed by Western ligand blotting using 12SI-IGF-I and/or 125I-IGF-II as the labeling ligand. This method is extremely useful in detecting IGFBPs; however, it requires the use of radioactive materials and usually takes several days to weeks to complete. Herein, we report on a modification of this method using biotinylated IGFs to detect both purified recombinant IGFBPs, as well as IGFBPs in fibroblast conditioned medium. This alternative method has proved sensitive, time- and cost-efficient, and it is environmentally safe.

A rapid, non-radioactive method for the detection of insulin-like growth factor binding proteins by Western ligand blotting.