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Genetically engineered mouse models through gene deletion are useful tools for analyzing gene function. To delete a gene in a certain tissue temporally, tissue-specific and tamoxifen-inducible Cre transgenic mice are generally used. Here, we generated transgenic mouse with cardiac-specific expression of Cre recombinase fused to a mutant estrogen ligand-binding domain (ERT2) on both N-terminal and C-terminal under the regulatory region of human vasoactive intestinal peptide receptor 2 ( VIPR2 ) intron and  Hsp68  promoter ( VIPR2-ERT2CreERT2 ). In  VIPR2-ERT2CreERT2  transgenic mice, mRNA for  Cre  gene was highly expressed in the heart. To further reveal heart-specific  Cre  expression,  VIPR2-ERT2CreERT2  mice mated with  ROSA26-lacZ  reporter mice were examined by X-gal staining. Results of X-gal staining revealed that Cre-dependent recombination occurred only in the heart after treatment with tamoxifen. Taken together, these results demonstrate that  VIPR2-ERT2CreERT2  transgenic mouse is a useful model to unveil a specific gene function in the heart.

Tamoxifen-inducible cardiac-specific Cre transgenic mouse using VIPR2 intron