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Background This study was carried out to investigate the antifungal activity of chitinase produced by  Streptomyces  species isolated from grassland soils against selected pathogenic fungi, namely:  Magnaporthe oryzae ,  Fusarium graminearum ,  Rhizoctonia solani ,  Puccinia  species and  Botyrtis cinerea . Chitinase produced by  Streptomyces albus  was purified consecutively with dialysis, gel filtration and ion- exchange chromatography, the test isolate can be exploited for large scale production of chitinase. Results Seven  Streptomyces  species ( Streptomyces rimosus, S. albus, S. riseoflavus, S. fumosus, S. spiralis, S. aureofaciens and S. flavogriseus)  isolated from three different grassland soils were screened based on the morphology and their ability to produce chitinase.  S .  rimosus ,  S .  fumosus ,  S .  albus  and  S .  flavogriseus  were able to produce chitinase, although the enzyme chitinase was found to be higher in  Streptomyces albus . The effect of environmental factors were determined on  Streptomyces albus  being the best chitinolytic producing isolate. Temperature of 30 ο C was favourable for  Streptomyces albus  and pH 6.0 was a growth factor for maximum chitinase production. The activity of the enzyme was stimulated by the addition of metal ions like Mn 2+ , K + , Na + , Mg + , Fe 2+ , Ca 2+  but Zn 2+  inhibited chitinase activity. Chitinase activity was favoured by maltose as carbon source and NH 4  (SO 4 ) 2  as nitrogen source. Culture filtrate of  Streptomyces albus  was able to inhibit the growth of all the selected pathogenic fungi namely  Magnaporthe oryzae ,  Fusarium graminearum ,  Rhizoctonia solani ,  Puccinia  species and  Botyrtis cinerea  whereas  Streptomyces rimosus  has the least inhibitory activity. Conclusions Streptomyces albus  has the highest ability to produce chitinase which can act as antifungal for degradation of the cell wall of many phytopathogenic fungi.

Antifungal activity of chitinase produced by Streptomyces species isolated from grassland soils in Futa Area, Akure