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Background Cabbage butterfly,  Pieris brassicae  Linnaeus (Lepidoptera: Pieridae), is a major insect pest affecting cole crops worldwide. Excessive applications of chemical-based insecticides have a devastating impression over the organisms and environment. Results In this study, entomopathogenic nematode (EPN)  Heterorhabditis bacteriophora  Poinar strain EUPT-S26 (local isolate) was evaluated for  Pieris brassicae  control under polyhouse and field conditions. Under the polyhouse conditions, the highest insect mortality 91.6 and 94.0% was observed in the plots treated with the nematodes suspension 1500 IJs/ml and 2000 IJs/ml, respectively. Based on the highest cabbage plant protection under polyhouse conditions,  H. bacteriophora  EUPT-S26 was also applied for field assays in the course of the crop’s productive phase. Data demonstrated from the field treatments signify the highest concentration (2000 IJs/ml) showed the maximum larval mortality and least damage percentage 45 ± 1.07% that remained constant until harvesting; this resulted in the highest productivity in polyhouse and under field conditions. Conclusion According to assessed field conditions, it was suggested to perform 3 applications of EPNs during the vegetative phase and at the time of head formation to increase productivity and to reduce damage. The results approved that EPNs are an effective alternative of chemical-based insecticides to control the cabbage butterfly.

Infectivity of entomopathogenic nematode against the cabbage butterfly (Pieris brassicae L.) in polyhouse and in field condition