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Background The expression of  FZD5  distinguishes immature human mesenchymal stem/stromal cells (MSC) in cultures, and the function of FZD5 is crucial for maintaining the proliferation and multilineage differentiation capacity of human MSC. We herein investigated whether  Fzd5  expression also marks undifferentiated MSC in animals. Methods We generated a transgenic mouse strain ( Fzd5-CreERT-tFP635 ) that expresses  CreERT  and the fluorescent protein,  TurboFP635  ( tFP635 ), under the transcriptional control of the  Fzd5  gene using the BAC transgenic technique, and identified cells expressing tFP635 by flow cytometry. We also conducted lineage tracing with this strain. Results In the bone marrow of transgenic mice, tFP635 was preferentially expressed in MSC, Leptin receptor-expressing MSC (LepR + MSCs), and some Pdgfrα +  Sca1 +  MSC (PαS). Inducible lineage tracing using the  Fzd5-CreERT-tFP635 ;  CAG-CAT-EGFP  strain at the adult stage showed that  Fzd5 -expressing cells and their descendants labeled with GFP were progressively dominant in LepR + MSC and PαS, and GFP +  cells persisted for 1 year after the activation of CreERT. Adipocyte progenitor cells (APCs), osteoblast progenitor cells (OPCs), and Cd51 +  stromal cells were also labeled with GFP. Conclusions Our transgenic mouse marks two different types of MSC, LepR + MSC and PαS.

Generation of a BAC transgenic mouse strain that expresses CreERT and a fluorescent protein under the transcriptional control of the Fzd5 locus