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Background  High-resolution melting analysis (HRMA) is a novel molecular technique based on the real-time PCR that can be used to detect vancomycin resistance Enterococcus (VRE). The purpose of this study was to identify VRE species with HRMA in clinical isolates.    Results  Out of 49 Enterococcus isolates, 11 (22.44%)  E. faecium  isolates and 19 (38.77%)  E. faecalis  isolates were detected. Average melting temperatures for  divIVA  in  E.faecalis ,  alanine racemase  in  E.faecium , and  vanA  in VRE strains were obtained as 79.9 ± 0.5 °C, 85.4 ± 0.5 °C, and 82.99 ± 0.5 °C, respectively. Furthermore, the data showed that the HRMA method was sensitive to detect 10 0  CFU/ml for the  divIVA ,  alanine racemase , and  vanA  genes. Also, out of 49 Enterococcus spp., which were isolated by HRMA assay, 8 isolates (16.32%) of  E. faecium  and 18 isolates (36.73%) of  E. faecalis  were detected. The  vanA  gene was reported in 2 isolates (25%) of  E. faecium  and 9 isolates (50%) of  E. faecalis .    Conclusions  This study demonstrated that using the HRMA method, we can detect  E. faecium ,  E. faecalis , and the  vanA  gene with high sensitivity and specificity.

tropical medicine and health

Development of high-resolution melting curve analysis in rapid detection of vanA gene, Enterococcus faecalis, and Enterococcus faecium from clinical isolates