English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Background   Vibrio cholerae  is associated with severe watery diarrheal disease among people in many parts of the world, including the coastal provinces of Southern Thailand. There are relatively few studies focusing on the genetic characterization among  V. cholerae  isolates in this region. Therefore, this study aimed at exploring the presence of virulence genes and DNA fingerprints among  V. cholerae  O1 and non - O1/non - O139 isolates obtained from clinical samples in four southern coastal provinces during the period of 2001 – 2009 ( n  = 21).    Results  All  V. cholerae  O1 isolates possessed  ctxA ,  tcpA ,  zot ,  ace ,  hlyA , and  vasH  genes. However, only  hlyA ,  vcsV2 , and  vasH  genes were detected in the majority of the non - O1/non - O139 isolates. All O1 isolates showed indistinguishable PCR fingerprints by arbitrarily primed (AP) - PCR and enterobacterial repetitive intergenic consensus (ERIC) - PCR regardless of the geographical area and period of isolation. However, the multi-locus variable-number of tandem-repeat analysis (MLVA) could differentiate these O1 isolates ( n  = 11) into eight profiles. Isolates exhibiting an undistinguished MLVA profile also showed identical pulsed-field gel electrophoresis (PFGE). In addition, the O1 isolates were grouped into the same cluster by all methods used in this study.    Conclusions  This study demonstrated the presence of virulence genes and genetic diversity among different serogroups of  V. cholerae  isolates from clinical samples in southern Thailand.  V. cholerae  O1 isolated over a period of multiple years were genetically related, suggesting that they had a clonal origin, whereas non - O1/non - O139 isolates could have evolved independently.

tropical medicine and health

Comparison of virulence gene profiles and genomic fingerprints of Vibrio cholerae O1 and non-O1/non-O139 isolates from diarrheal patients in southern Thailand