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Background  Chromosome microdissection is one of the most important techniques in molecular cytogenetic research. Cotton ( Gossypium  Linnaeus, 1753) is the main natural fiber crop in the world. The resistance gene analog (RGA) cloning after its single chromosome microdissection can greatly promote cotton genome research and breeding.    Results  Using the linker adaptor PCR (LA-PCR) with the primers of rice disease-resistance homologues, three nucleotide sequences PS016 (KU051681), PS054 (KU051682), and PS157 (KU051680) were obtained from the chromosome A h 01 of upland cotton (cv. TM-1). The Blast results showed that the three sequences are the nucleotide binding site-leucine rich repeat (NBS-LRR) type RGAs. Clustering results indicated that they are homologous to these published RGAs. Thus, the three RGAs can definitely be confirmed as NBS-LRR class of RGAs in upland cotton.    Conclusions  Using single chromosome microdissection technique, DNA libraries containing cotton RGAs were obtained. This technique can promote cotton gene cloning, marker development and even the improvement of cotton genome research and breeding.    Electronic supplementary material  The online version of this article (doi:10.1186/s41065-017-0035-3) contains supplementary material, which is available to authorized users.

Microdissection of the Ah01 chromosome in upland cotton and microcloning of resistance gene anologs from the single chromosome