English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Phosphoglucose isomerase (PGI, EC 5.3.1.9) is an essential metabolic enzyme in all eukaryotes. An earlier study of the  PgiC1  gene, which encodes cytosolic PGI in the grass  Festuca ovina  L., revealed a marked difference in the levels of nucleotide polymorphism between the 5’ and 3’ portions of the gene.    Methods  In the present study, we characterized the sequence polymorphism in  F. ovina PgiC1  in more detail and examined possible explanations for the non-uniform pattern of nucleotide polymorphism across the gene.    Results  Our study confirms that the two portions of the  PgiC1  gene show substantially different levels of DNA polymorphism and also suggests that the peptide encoded by the 3’ portion of  PgiC1  is functionally and structurally more important than that encoded by the 5’ portion. Although there was some evidence of purifying selection ( d   N / d   S  test) on the 5’ portion of the gene, the signature of purifying selection was considerably stronger on the 3’ portion of the gene ( d   N / d   S  and McDonald–Kreitman tests). Several tests support the action of balancing selection within the 5’ portion of the gene. Wall’s  B  and  Q  tests were significant only for the 5’ portion of the gene. There were also marked peaks of nucleotide diversity, Tajima’s  D  and the  d   N / d   S  ratio at or around a  PgiC1  codon site (within the 5’ portion of the gene) that a previous study had suggested was subject to positive diversifying selection.    Conclusions  Our results suggest that the two portions of the gene have been subject to different selective regimes. Purifying selection appears to have been the main force contributing to the relatively low level of polymorphism within the 3’ portion of the sequence. In contrast, it is possible that balancing selection has contributed to the maintenance of the polymorphism within the 5’ portion of the gene.    Electronic supplementary material  The online version of this article (doi:10.1186/s41065-017-0032-6) contains supplementary material, which is available to authorized users.

Contrasting patterns of nucleotide polymorphism suggest different selective regimes within different parts of the PgiC1 gene in Festuca ovina L.