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Background  The  supF  gene of  Escherichia coli  is useful for forward mutation analysis in bacterial and mammalian cells used in mutagenesis and DNA repair studies. Indicator  E. coli  strains, such as KS40/pOF105, have been used to analyze  supF  mutations. However, KS40/pOF105 is not enough to select  supF  mutants on nutrient-rich agar plates. Therefore, in this study, a new indicator  E. coli  strain for rapid and accurate detection of  supF  mutations was developed.    Results  The  gyrA  and  rpsL  genes with an amber mutation were integrated into the chromosomal DNA of  E. coli  KS40 to produce a new indicator strain, RF01. RF01 cells transformed by the wild-type  supF  gene were sensitive to nalidixic acid and streptomycin on LB agar plates.  supF  mutant frequencies and mutation spectra in RF01 were similar to those in KS40/pOF105. In addition, some mutations in  supF  were only detected in RF01.    Conclusion  RF01 is a new and useful indicator  E. coli  strain for analyzing  supF  mutations.

New indicator Escherichia coli strain for rapid and accurate detection of supF mutations