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To the editor, Rapid identification of causative pathogenic bacteria is crucial for the treatment of patients with infectious diseases, including pneumonia [1]. For this purpose, molecular techniques of genetic diagnosis of infectious diseases have been developed and applied to rapid diagnostic procedures [2, 3]. In this report, we conducted 16S ribosomal RNA (rRNA) gene amplicon sequencing and metagenomic analysis of DNA extracted from airway secretion of a patient suffering from acute respiratory distress syndrome (ARDS) as a consequence of pneumonia by using the portable DNA sequencer MinIONTM [4, 5] (see Additional file 1).

Real-time diagnostic analysis of MinION™-based metagenomic sequencing in clinical microbiology evaluation: a case report