English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

Background    Pleurotus sapidus  secretes a huge enzymatic repertoire including hydrolytic and oxidative enzymes and is an example for higher basidiomycetes being interesting for biotechnology. The complex growth media used for submerged cultivation limit basic physiological analyses of this group of organisms. Using undefined growth media, only little insights into the operation of central carbon metabolism and biomass formation,  i.e. , the interplay of catabolic and anabolic pathways, can be gained.    Results  The development of a chemically defined growth medium allowed rapid growth of  P. sapidus  in submerged cultures. As  P. sapidus  grew extremely slow in salt medium, the co-utilization of amino acids using  13 C-labelled glucose was investigated by gas chromatography–mass spectrometry (GC-MS) analysis. While some amino acids were synthesized up to 90%  in vivo  from glucose ( e.g.,  alanine), asparagine and/or aspartate were predominantly taken up from the medium. With this information in hand, a defined yeast free salt medium containing aspartate and ammonium nitrate as a nitrogen source was developed. The observed growth rates of  P. sapidus  were well comparable with those previously published for complex media. Importantly, fast growth could be observed for 4 days at least, up to cell wet weights (CWW) of 400 g L -1 . The chemically defined medium was used to carry out a  13 C-based metabolic flux analysis, and the  in vivo  reactions rates in the central carbon metabolism of  P. sapidus  were investigated. The results revealed a highly respiratory metabolism with high fluxes through the pentose phosphate pathway and TCA cycle.    Conclusions  The presented chemically defined growth medium enables researchers to study the metabolism of  P. sapidus , significantly enlarging the analytical capabilities. Detailed studies on the production of extracellular enzymes and of secondary metabolites of  P. sapidus  may be designed based on the reported data.    Electronic supplementary material  The online version of this article (doi:10.1186/s40694-014-0009-4) contains supplementary material, which is available to authorized users.

A minimal growth medium for the basidiomycete Pleurotus sapidus for metabolic flux analysis