English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Theoretically human embryonic stem cells (hESCs) have the capacity to self-renew and differentiate into all human cell types. Therefore, the greatest promise of hESCs-based therapy is to replace the damaged tissues of patients suffering from traumatic or degenerative diseases by the exact same type of cells derived from hESCs. Allograft immune rejection is one of the obstacles for hESCs-based clinical applications. Human leukocyte antigen (HLA) II leads to CD4 +  T cells-mediated allograft rejection. Hence, we focus on optimizing hESCs for clinic application through gene modification.    Results  Transcription activator-like effector nucleases (TALENs) were used to target MHC class II transactivator ( CIITA ) in hESCs efficiently.  CIITA  −/−  hESCs did not show any difference in the differentiation potential and self-renewal capacity. Dendritic cells (DCs) derived from  CIITA  −/−  hESCs expressed CD83 and CD86 but without the constitutive HLA II. Fibroblasts derived from  CIITA  −/−  hESCs were powerless in IFN-γ inducible expression of HLA II.    Conclusion  We generated HLA II defected hESCs via deleting  CIITA , a master regulator of constitutive and IFN-γ inducible expression of HLA II genes.  CIITA  −/−  hESCs can differentiate into tissue cells with non-HLA II expression. It’s promising that  CIITA  −/−  hESCs-derived cells could be used in cell therapy (e.g., T cells and DCs) and escape the attack of receptors’ CD4 +  T cells, which are the main effector cells of cellular immunity in allograft.    Electronic supplementary material  The online version of this article (doi:10.1186/s40659-015-0051-6) contains supplementary material, which is available to authorized users.

Functional disruption of human leukocyte antigen II in human embryonic stem cell